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Quality control of the PCR products and their in vitro transcripts. (A) Size fractionation of purified PCR product (100 ng) on 3% agarose gel stained with GelRed. The expected sizes for tagged or untagged sequences are 86 bp or 65 bp, respectively (arrowheads). (B) Agarose gel electrophoresis of the indicated in vitro transcripts (250 ng) following column purification. A minor species of untagged RNA (<10%) migrated more slowly than the main product, likely due to alternative folding.

Journal: Frontiers in Molecular Biosciences

Article Title: FluoTag-EMSA: a fast and accessible quantitative method to assess RNA-binding specificity using 3′-tagged hybrid duplexes

doi: 10.3389/fmolb.2025.1727371

Figure Lengend Snippet: Quality control of the PCR products and their in vitro transcripts. (A) Size fractionation of purified PCR product (100 ng) on 3% agarose gel stained with GelRed. The expected sizes for tagged or untagged sequences are 86 bp or 65 bp, respectively (arrowheads). (B) Agarose gel electrophoresis of the indicated in vitro transcripts (250 ng) following column purification. A minor species of untagged RNA (<10%) migrated more slowly than the main product, likely due to alternative folding.

Article Snippet: AgaPureTM Agarose LE (Canvax, CANAG006) 10× TAE (Tris-Acetate-EDTA) buffer: 0.4 M Tris, 0.2 M acetic acid, 10 mM EDTA, pH ∼8.3 GelRed (Biotium, 41,001) Gel Loading Dye Purple 6X (NEB, B7024S) Horizontal agarose gel electrophoresis system (VWR 700-0082) Standard Power Pack Power Supply (Biometra, P25) Bio-Rad ChemiDocTM Imager For EMSA.

Techniques: Control, In Vitro, Fractionation, Purification, Agarose Gel Electrophoresis, Staining